| Expression pattern: |
UP |
| Associated gene: |
MDM2, p53, RUNX3, LPAR1 |
| Associated microRNA: |
miR-496 |
| Biological function: |
Promotes osteogenic differentiation of ADSCs; increases osteogenic markers (Runx2, ALP, Osx, OCN) and decreases p53. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-496 to relieve repression of MDM2, thereby inhibiting p53 and promoting osteogenic differentiation; RUNX3 binds the LPAR1 promoter to increase hsa_circ_0005752 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); p53 signaling (inhibits); proliferation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Provided a new therapeutic strategy for osteoporosis. |
| Description: |
In OM-induced ADSC osteogenesis (osteoporosis-relevant context), hsa_circ_0005752 is up-regulated and promotes osteogenic differentiation. It functions as a ceRNA that sponges miR-496 to increase MDM2 and suppress p53, and its expression is transcriptionally enhanced by RUNX3 via the LPAR1 promoter. |
| Confidence score: |
0.6145 |