| Expression pattern: |
UP |
| Associated gene: |
KPNA4 |
| Associated microRNA: |
miR-548I |
| Biological function: |
Promotes NSCLC cell viability/proliferation, migration, invasion and angiogenesis; inhibits apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-548I to regulate KPNA4 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Transwell Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
NSCLC patients with high circ_0008003 expression had low survival rates in comparison with the patients with low circ_0008003 expression. |
| Description: |
hsa_circ_0008003 is up-regulated in NSCLC tissues and cell lines and its high expression is associated with poorer overall survival. Functionally, it promotes NSCLC malignant phenotypes and tumor growth by acting as a ceRNA to sponge miR-548I, thereby increasing KPNA4 expression. |
| Confidence score: |
0.8068 |