| Expression pattern: |
DN |
| Associated gene: |
PIF1, AGO2, AIM2, ASC, BRCA1, gammaH2AX, caspase-1, GSDMD, GSDMD-N, IL-1beta, IL-18 |
| Associated microRNA: |
miR-1184 |
| Biological function: |
circNEIL3 knockdown promotes radiation-induced cell pyroptosis and enhances radiotherapy efficacy; circNEIL3 overexpression inhibits pyroptosis in irradiated LUAD cells. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-1184 to relieve repression of PIF1, leading to increased DNA damage and AIM2 inflammasome activation, thereby affecting pyroptosis during radiotherapy. |
| Biological pathway or process: |
pyroptosis (inhibits); other pathway/process (other); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Transfection; ELISA; Annexin V/PI Flow Cytometry; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); Colony Formation Assay; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target/strategy to enhance radiotherapy in lung cancer. |
| Description: |
In irradiated LUAD cells, circNEIL3 is downregulated and functions as a cytoplasmic ceRNA by sponging miR-1184 to upregulate PIF1. This axis modulates DNA damage/AIM2 inflammasome signaling to regulate radiation-induced pyroptosis and affects radiotherapy efficacy in vivo. |
| Confidence score: |
0.8366 |