| Expression pattern: |
UP |
| Associated gene: |
caprin1, NR3B, NMDA receptor 3B |
| Associated microRNA: |
- |
| Biological function: |
Protects against epilepsy-induced neuronal injury, promotes hippocampal neurogenesis, inhibits abnormal migration of newborn neurons, reduces epileptiform discharges and spontaneous seizures, and alleviates mood disorders and cognitive deficits in epileptic mice. |
| Molecular mechanism: |
circ288 directly binds the RNA-binding protein caprin1 and inhibits its degradation, increasing caprin1 protein stability; circ288/caprin1 increases NR3B mRNA levels indirectly, implicating a circ288-caprin1-NR3B pathway. |
| Biological pathway or process: |
migration (inhibits); mRNA stability (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Clinical Sample Validation; Bioinformatics Analysis; Transfection; RIP (RNA Immunoprecipitation); Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
circ288 was identified in serum of epileptic patients in remission and is proposed as a promising candidate for anti-epileptic RNA therapy. |
| Description: |
This study identifies human circ288/hsa_circ_0000288 as a serum circRNA elevated in epileptic patients in remission and as a candidate therapeutic RNA for epilepsy. In mouse and in vitro epilepsy models, circ288 protects neurons, supports hippocampal neurogenesis, reduces aberrant neuronal migration, suppresses epileptogenesis-related seizures and discharges, and improves cognitive and mood deficits. Mechanistically, circ288 directly binds and stabilizes the RBP caprin1, leading to increased NR3B mRNA levels through a proposed circ288-caprin1-NR3B pathway; exosome-encapsulated circ288 also shows anti-epileptic activity. |
| Confidence score: |
0.782 |