| Expression pattern: |
UP |
| Associated gene: |
ZEB2 |
| Associated microRNA: |
miR-342-3p |
| Biological function: |
Promotes proliferation, migration and invasion and inhibits apoptosis in NSCLC cells; promotes xenograft tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-342-3p to positively regulate ZEB2 expression (ceRNA-like mechanism). |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circVAPA expression is associated with poor overall survival and disease-free survival; shows diagnostic value in serum (AUC 0.9278). |
| Description: |
circVAPA is upregulated in NSCLC tissues/cell lines and promotes malignant phenotypes (proliferation, migration/invasion, EMT) while inhibiting apoptosis. Mechanistically, it sponges miR-342-3p to increase ZEB2 expression, and its knockdown suppresses xenograft tumor growth; high circVAPA correlates with poor prognosis and shows serum diagnostic potential. |
| Confidence score: |
0.8229 |