| Expression pattern: |
UP |
| Associated gene: |
Bcl-2, cleaved caspase-3 |
| Associated microRNA: |
miR-136-5p |
| Biological function: |
hsa_circ_0014130 functions as an oncogenic circRNA and tumor promoter in NSCLC, promoting proliferation, invasion and tumor growth while inhibiting apoptosis. |
| Molecular mechanism: |
hsa_circ_0014130 acts as a ceRNA/miRNA sponge for miR-136-5p, thereby upregulating Bcl-2 and regulating apoptosis-related cleaved caspase-3. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; FISH / smFISH; Western Blot; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0014130 expression could discriminate NSCLC tissues from adjacent normal tissues, correlated with tumor size, lymphatic metastasis, distant metastasis and TNM stage, and was associated with poor prognosis; it might function as a prognostic factor, biomarker and therapeutic target in NSCLC. |
| Description: |
hsa_circ_0014130 is up-regulated in NSCLC tissues and acts as an oncogenic circRNA. It promotes NSCLC cell proliferation, invasion and xenograft tumor growth, at least partly by sponging miR-136-5p and increasing Bcl-2 expression to inhibit apoptosis. Its expression has diagnostic/prognostic relevance and may serve as a biomarker or therapeutic target. |
| Confidence score: |
0.7369 |