| Expression pattern: |
DN |
| Associated gene: |
PHLPP2, AKT, cyclin D1, transforming growth factor-beta, STAT5, CREB, AGO2, ZIP4 |
| Associated microRNA: |
miR-373 |
| Biological function: |
Inhibits pancreatic cancer cell proliferation and tumor growth; ameliorates cancer-associated cachexia by inhibiting muscle wasting. |
| Molecular mechanism: |
Acts as a miR-373 sponge to increase PHLPP2, leading to AKT dephosphorylation and down-regulation of cyclin D1; reduces STAT5 phosphorylation to decrease TGF-beta expression/secretion; participates in a CREB-miR-373-PHLPP2 feed-forward loop. |
| Biological pathway or process: |
ceRNA regulation (promotes); PI3K/AKT (inhibits); proliferation (inhibits); cell cycle (inhibits); apoptosis (other); inflammation (other); fibrosis (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; EdU Staining; Colony Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; ELISA; Western Blot |
| Clinical significance: |
circANAPC7 level was significantly lower in human pancreatic cancer tissues than tumor-adjacent tissues. |
| Description: |
circANAPC7 is down-regulated in pancreatic cancer and functions as a tumor suppressor and anti-cachexia factor. It sponges miR-373 to de-repress PHLPP2, leading to reduced AKT phosphorylation and decreased cyclin D1-driven proliferation. Through the PHLPP2/AKT/STAT5 axis it reduces TGF-beta expression/secretion, alleviating muscle wasting, and participates in a CREB-miR-373-PHLPP2 feed-forward loop linked to ZIP4 signaling. |
| Confidence score: |
0.8794 |