| Expression pattern: |
DN |
| Associated gene: |
FOXO4 |
| Associated microRNA: |
miR-532-3p |
| Biological function: |
Acts as a tumor suppressor in colorectal cancer; knockdown promotes colorectal cancer cell proliferation and migration, promotes G0/G1-to-S phase transition, and inhibits apoptosis. |
| Molecular mechanism: |
ceRNA mechanism involving competitive binding to miR-532e3p and regulation of FOXO4 expression through the hsa_circRNA_103809/miR-532e3P/FOXO4 axis. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Low expression is associated with tumor stage and lymph node metastasis; may be a potential biomarker and target for diagnosis, treatment, and prognosis of colorectal cancer. |
| Description: |
hsa_circRNA_103809/hsa_circ_0072088 is down-regulated in colorectal cancer tissues and cell lines and is associated with clinical stage and lymph node metastasis. Functionally, loss of hsa_circRNA_103809 promotes CRC cell proliferation and migration, while mechanistically it may act as a ceRNA for miR-532e3p to regulate FOXO4 expression. The study suggests hsa_circRNA_103809 is a tumor-suppressive circRNA and a potential biomarker or therapeutic target in CRC. |
| Confidence score: |
0.5761 |