| Expression pattern: |
UP |
| Associated gene: |
TFAM, ND1, ATP6 |
| Associated microRNA: |
miR-1182 |
| Biological function: |
promotes proliferation and invasion; inhibits apoptosis; alleviates mtDNA damage; promotes tumor growth; transported via exosomes |
| Molecular mechanism: |
circ_0002476 sponges miR-1182 to positively regulate TFAM, affecting mtDNA damage and malignant phenotypes; exosome-mediated intercellular transport |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); mitochondrial function (other); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_0002476 was associated with lower overall survival in patients with NSCLC; correlated with TNM grade, lymph node metastasis and tumor size |
| Description: |
circ_0002476 is up-regulated in NSCLC and mainly localized in the cytoplasm. It promotes NSCLC proliferation, invasion and tumor growth while inhibiting apoptosis and mtDNA damage by sponging miR-1182 to upregulate TFAM, and it can be transported between cells via exosomes. |
| Confidence score: |
0.8428 |