| Expression pattern: |
UP |
| Associated gene: |
CTPS1, Notch1, Hes1, Numb, E-cadherin, N-cadherin, vimentin |
| Associated microRNA: |
let-7b-5p |
| Biological function: |
promotes breast cancer cell proliferation, invasion, migration and epithelial-mesenchymal transition; promotes tumor growth in vivo |
| Molecular mechanism: |
circ_0000437 acts as a molecular sponge for let-7b-5p to regulate CTPS1 expression and promote malignant phenotypes and EMT; CTPS1 regulates the Notch signaling pathway |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); migration (promotes); EMT (promotes); Notch (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circ_0000437 is upregulated in breast cancer tissues and cell lines. It promotes proliferation, invasion, migration, EMT and in vivo tumor growth by sponging let-7b-5p and regulating CTPS1, with downstream involvement of the Notch signaling pathway. |
| Confidence score: |
0.7795 |