| Expression pattern: |
UP |
| Associated gene: |
CLIC1 |
| Associated microRNA: |
miR-548c-3p |
| Biological function: |
promotes gastric cancer cell viability, proliferation, migration, invasion, tumorigenesis, metastasis and immune escape; impairs CD8+ T cell function and promotes CD8+ T cell apoptosis through CLIC1 |
| Molecular mechanism: |
circ_0008287 acts as a ceRNA sponge for miR-548c-3p, relieving miR-548c-3p-mediated inhibition of CLIC1 and thereby promoting CLIC1 expression and immune escape |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (promotes); immune regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Bioinformatics Analysis; Survival Analysis; Transfection; CCK8; Wound Healing Assay; Transwell Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; FISH / smFISH; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; Annexin V/PI Flow Cytometry; In Vivo Animal Model |
| Clinical significance: |
high circ_0008287 expression is associated with shorter overall survival, poor prognosis, tumor size, clinical cancer stage, and lymph node metastasis in gastric cancer |
| Description: |
circ_0008287 is up-regulated in gastric cancer and promotes malignant phenotypes including proliferation, invasion, migration, tumorigenesis and metastasis. Mechanistically, it sponges miR-548c-3p to increase CLIC1 expression, impairing CD8+ T cell function and promoting T cell apoptosis, thereby enhancing immune escape. Higher circ_0008287 expression is associated with poorer overall survival and aggressive clinicopathological features. |
| Confidence score: |
0.7421 |