| Expression pattern: |
DS |
| Associated gene: |
AKT1, PIK3CG, TGFBR2, MAPK1 |
| Associated microRNA: |
hsa-miR-196b-5p, hsa-miR-451a |
| Biological function: |
May play a regulatory role in prostate cancer cells in the EMT induction model by indirectly regulating PI3K-Akt, TGF-beta, and MAPK signaling-related proteins through miRNAs. |
| Molecular mechanism: |
Predicted circRNA-miRNA-mRNA/protein regulatory network involving hsa-miR-196b-5p and hsa-miR-451a and downstream PI3K-Akt, TGF-beta, and MAPK signaling components. |
| Biological pathway or process: |
PI3K/AKT (other); TGF-beta/SMAD (other); MAPK (other); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; circRNA-seq; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
hsa_circ_0001085 was differentially expressed in an IFN-gamma-induced prostate cancer EMT model and validated by qPCR. The study predicted that it may regulate PI3K-Akt, TGF-beta, and MAPK signaling through hsa-miR-196b-5p and hsa-miR-451a, suggesting a role in EMT-related prostate cancer cell regulation. |
| Confidence score: |
0.4943 |