| Expression pattern: |
UP |
| Associated gene: |
IGF2BP3, TRIM25, CCND1 |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation of gastric cancer cells in vitro and in vivo |
| Molecular mechanism: |
CircNFATC3 binds IGF2BP3 in the cytoplasm and prevents TRIM25-mediated ubiquitination to stabilize IGF2BP3 protein, enhancing IGF2BP3-CCND1 axis and promoting CCND1 mRNA stability. |
| Biological pathway or process: |
proliferation (promotes); mRNA stability (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
H
|
| Validation methods: |
circRNA-seq; RIP (RNA Immunoprecipitation); Sanger Sequencing; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); RNA Pull-Down; IF (Immunofluorescence); Co-IP; Transfection; CCK8; Colony Formation Assay; EdU Staining; In Vivo Animal Model; IHC (Immunohistochemistry); Actinomycin D / DRB Stability Assay; Western Blot |
| Clinical significance: |
CircNFATC3 was positively associated with tumor volume. |
| Description: |
CircNFATC3 is upregulated in gastric cancer and promotes tumor cell proliferation. It binds IGF2BP3 in the cytoplasm, inhibits TRIM25-mediated ubiquitination to stabilize IGF2BP3, and thereby enhances CCND1 mRNA stability. |
| Confidence score: |
0.8467 |