| Expression pattern: |
UP |
| Associated gene: |
XIAP |
| Associated microRNA: |
miR-192, miR-215 |
| Biological function: |
Promotes hepatocellular carcinoma cell proliferation, migration, invasion, metastasis, and tumor growth; functions as an oncogenic ceRNA. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-192 and miR-215 to regulate XIAP expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; circRIP; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Actinomycin D / DRB Stability Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
High circKIF5B expression is associated with worse overall survival and recurrence-free survival, and correlates with histological grade and pathological stage in liver cancer patients. |
| Description: |
circKIF5B is up-regulated in hepatocellular carcinoma tissues and cell lines and promotes malignant phenotypes (proliferation, migration/invasion, metastasis, tumor growth). Mechanistically, it acts as a ceRNA that sponges miR-192 and miR-215 to increase XIAP expression, and high circKIF5B levels are associated with worse patient outcomes. |
| Confidence score: |
0.887 |