| Expression pattern: |
DS |
| Associated gene: |
FOXO3 |
| Associated microRNA: |
miR-23a |
| Biological function: |
Knockdown of circRNA_FOXO3 restores LPS-induced cell viability loss and cytokine dysregulation in HUVECs (anti-inflammatory effect in this in vitro sepsis-like model). |
| Molecular mechanism: |
miR-23a binds and suppresses circRNA_FOXO3 (luciferase reporter); circRNA_FOXO3 is involved in the miR-23a/FOXO3 regulatory network affecting inflammatory cytokine responses under LPS stimulation. |
| Biological pathway or process: |
inflammation (inhibits); immune regulation (other); proliferation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Luciferase Reporter Assay; Transfection; ELISA; MTT |
| Clinical significance: |
Genotypes associated with lower circRNA_FOXO3 (rs12196996 GG with rs2232365 AA) correlate with prolonged survival and suppressed severity in ICU-acquired sepsis. |
| Description: |
In ICU-acquired sepsis patient samples, circRNA_FOXO3 expression differs by rs12196996/rs2232365 genotype combinations. Mechanistically, miR-23a suppresses circRNA_FOXO3 (and FOXO3), and circRNA_FOXO3 knockdown alleviates LPS-induced loss of viability and pro-inflammatory cytokine dysregulation in HUVECs, linking this axis to sepsis severity and survival. |
| Confidence score: |
0.6762 |