| Expression pattern: |
UP |
| Associated gene: |
YBX1, BRCC36, p62, AGO2, HIF-1alpha |
| Associated microRNA: |
- |
| Biological function: |
Promotes HCC cell proliferation, migration, invasion and tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
Binds YBX1 and recruits BRCC36 to deubiquitinate (K63-linked) and stabilize YBX1, preventing p62-mediated selective autophagic degradation via AMPK/mTOR; activates HIF-1 signaling pathway. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); autophagy (inhibits); AMPK (inhibits); PI3K/AKT/mTOR (other); HIF-1 signaling (promotes); ubiquitination (inhibits); selective autophagy (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; FISH / smFISH; divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
Upregulated in HCC tissues and serum exosomes; associated with advanced tumor stages (Stage III-IV) and poor prognosis. |
| Description: |
circZNF79(5) is upregulated in HCC tissues and serum exosomes and is mainly localized in the cytoplasm. It promotes HCC proliferation/migration/invasion and inhibits apoptosis by binding YBX1 and recruiting BRCC36 to remove K63-linked ubiquitin chains, stabilizing YBX1 and enhancing downstream HIF-1 signaling, while preventing p62-mediated selective autophagic degradation of YBX1 via AMPK/mTOR. |
| Confidence score: |
0.8933 |