| Expression pattern: |
UP |
| Associated gene: |
DAXX, ERK1/2, p-ERK, AGO2 |
| Associated microRNA: |
hsa-miR-188-5p, hsa-miR-623, hsa-miR-520f-3p, hsa-miR-127-5p, hsa-miR-146a-3p, hsa-miR-488-5p, hsa-miR-670-3p, hsa-miR-128-1-5p, hsa-miR-128-2-5p |
| Biological function: |
promotes proliferation, migration, and invasion of gastric cancer cells; promotes tumor growth in vivo; enhances drug resistance/survival under treatment |
| Molecular mechanism: |
acts as a ceRNA/miRNA sponge for hsa-miR-188-5p to regulate DAXX; interacts with ERK1/2 and p-ERK and modulates MAPK signaling and inhibitor response |
| Biological pathway or process: |
MAPK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Sanger Sequencing; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circRNA-0044301 expression level was significantly associated with a poor prognosis of GC patients |
| Description: |
In gastric cancer, hsa_circ_0044301 is up-regulated and promotes malignant phenotypes (proliferation, migration, invasion) and tumor growth. Mechanistically, it functions as a ceRNA by sponging hsa-miR-188-5p to regulate DAXX and is linked to MAPK/ERK1/2 signaling, also modulating sensitivity to GDC-0994 and 5-FU. High expression is associated with poorer patient prognosis. |
| Confidence score: |
0.8634 |