circRNA basic information
circBase ID: -
Name: hsa_circ_ATAD1
Synonym: Circ-ATAD1
Host Gene: ATAD1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues (OS); non-tumor tissues; normal bone tissues
Cell lines:

Hs 3.T; MG-63; Hfob1.19
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: premature miR-154-5p, PIWIL1
Associated microRNA: miR-154-5p
Biological function: increases osteosarcoma cell invasion and migration; attenuates the tumor-suppressive role of miR-154-5p
Molecular mechanism: circ-ATAD1 directly interacts with premature miR-154-5p and suppresses miR-154-5p maturation, leading to reduced mature miR-154-5p and increased invasion/migration
Biological pathway or process:

migration (promotes); invasion (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Transfection; Transwell Assay; Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

-
Description:

Circ-ATAD1 is up-regulated in osteosarcoma tissues and cell lines and promotes invasion and migration. Mechanistically, it localizes to both nucleus and cytoplasm, binds premature miR-154-5p, and suppresses miR-154-5p maturation, reducing mature miR-154-5p activity and affecting downstream targets such as PIWIL1.
Confidence score:

0.6432
Other information
Title:

Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration.

Journal: Journal of orthopaedic surgery and research
Published: 2021
PubMed ID: 34857012
Study type:

combined biological and clinical study
Data availability: The data are not publicly available due to their containing information that could compromise the privacy of research participants, but are available on reasonable request from the corresponding author.
Code availability: -