circRNA basic information
circBase ID: hsa_circ_0007159
Name: hsa_circ_ATG4B
Synonym: circATG4B / circ_0 007159
Host Gene: ATG4B
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: Colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; patient tissues; tumor
Cell lines:

HCT116; SW480; HT29; DLD1; HCT116-L-OHP; SW480-L-OHP; HT29-L-OHP; DLD1-L-OHP; HEK-293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TMED10, ATG4B, AGO2
Associated microRNA: -
Biological function: induces oxaliplatin resistance and decreases chemosensitivity by promoting autophagy (via exosomal transfer).
Molecular mechanism: circATG4B encodes a protein (circATG4B-222aa) that competitively binds TMED10, preventing TMED10 from binding ATG4B, thereby increasing autophagy and inducing chemoresistance.
Biological pathway or process:

autophagy (promotes); chemoresistance (promotes); drug resistance (promotes)
Detected method:
Q
Validation methods:

divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; IF (Immunofluorescence); Western Blot; TEM; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circATG4B expression is associated with worse disease-free survival and reduced benefit from oxaliplatin treatment in CRC patients.
Description:

circATG4B is up-regulated in oxaliplatin-resistant CRC (notably enriched in exosomes) and is associated with poorer DFS under oxaliplatin treatment. Mechanistically, circATG4B promotes oxaliplatin resistance by promoting autophagy, largely via encoding circATG4B-222aa that binds TMED10 and relieves TMED10-mediated inhibition of ATG4B.
Confidence score:

0.8557
Other information
Title:

A Novel Protein Encoded by Exosomal CircATG4B Induces Oxaliplatin Resistance in Colorectal Cancer by Promoting Autophagy.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Published: 2022
PubMed ID: 36285810
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available in the supplementary material of this article.
Code availability: -