circRNA basic information
circBase ID: hsa_circ_0014717
Name: -
Synonym: circ_0014717
Host Gene: -
Genomic location(hg19): chr1:156290629-156304709:-
Genomic location(hg38): chr1:156320838-156334918:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; tumor-adjacent tissues; tumor tissues

Cell lines:

LO2; HepG2; Hep3B; Huh7; SMMC-7721; MHCC97L

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: BTG2
Associated microRNA: miR-668-3p
Biological function: Suppresses HCC cell growth/proliferation, migration, invasion and tumorigenesis; promotes apoptosis; induces G1 cell-cycle arrest.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-668-3p to regulate BTG2 in HCC.
Biological pathway or process:

ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (inhibits)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Survival Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis

Clinical significance:

Low circ_0014717 expression levels were associated with significantly reduced overall survival and an increased risk of tumor recurrence; down-regulated circ_0014717 expression level was correlated with tumor size, TNM stage and metastasis.

Description:

circ_0014717 is down-regulated in HCC tissues and cell lines and functions as a tumor suppressor. Mechanistically, it sponges miR-668-3p to relieve repression of BTG2, thereby inhibiting proliferation/migration/invasion and suppressing xenograft tumor growth; higher circ_0014717 expression is associated with better survival outcomes.

Confidence score:

0.7795

Other information
Title:

Circular RNA circ_0014717 Suppresses Hepatocellular Carcinoma Tumorigenesis Through Regulating miR-668-3p/BTG2 Axis.

Journal: Frontiers in oncology
Published: 2020
PubMed ID: 33598424
Study type:

combined biological and clinical study

Data availability: The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
Code availability: -