circRNA basic information
circBase ID: hsa_circ_0001326
Name: hsa_circ_PHLDB2
Synonym: circ_ 0001326
Host Gene: PHLDB2
Genomic location(hg19): chr3:111632165-111639266:+
Genomic location(hg38): chr3:111913318-111920419:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004979
MONDO name: asthma
Disease details: asthma
Disease DO ID:
 2841
Disease MeSH ID:
 D001249
Disease NCIt ID:
 C28397
Disease ICD11 ID:
 1656445230
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

THP-1
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: USP4
Associated microRNA: hsa-miR-136-5p
Biological function: Promotes M2 macrophage polarization and suppresses M1 macrophage polarization under luteolin stimulation; its knockdown reverses luteolin-induced polarization changes.
Molecular mechanism: Acts as a ceRNA by interacting with hsa-miR-136-5p to regulate USP4 expression; supported by rescue experiments and dual-luciferase reporter assays.
Biological pathway or process:

macrophage polarization (other); inflammation (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; Transfection; Western Blot; ELISA; Luciferase Reporter Assay
Clinical significance:

-
Description:

In THP-1-derived macrophages, luteolin upregulates hsa_circ_0001326, which is required for luteolin-induced polarization changes. hsa_circ_0001326 modulates macrophage polarization through the hsa-miR-136-5p/USP4 axis, supported by knockdown, inhibitor rescue, and luciferase assays.
Confidence score:

0.5416
Other information
Title:

Luteolin activates M2 macrophages and suppresses M1 macrophages by upregulation of hsa_circ_0001326 in THP-1 derived macrophages.

Journal: Bioengineered
Published: 2022
PubMed ID: 35152837
Study type:

biological research
Data availability: -
Code availability: -