circRNA basic information
circBase ID: hsa_circ_0044516
Name: -
Synonym: circ_0044516
Host Gene: -
Genomic location(hg19): chr17:48263677-48271402:-
Genomic location(hg38): chr17:50186316-50194041:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

AGS; MKN-45; HGC-27; NCI-N87; GES-1; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HuR, AGO2
Associated microRNA: miR-149-5p
Biological function: promotes GC cell proliferation, viability, colony formation, migration, invasion and in vivo tumor growth
Molecular mechanism: circ_0044516 acts as a miR-149-5p sponge to modulate HuR expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis
Clinical significance:

circ_0044516 could be a useful therapeutic target in GC in future
Description:

circ_0044516 is up-regulated in gastric cancer cell lines and promotes malignant phenotypes, including proliferation/viability, colony formation, migration, invasion and xenograft tumor growth. Mechanistically, cytoplasmic circ_0044516 functions as a sponge for miR-149-5p to increase HuR expression, forming a circ_0044516/miR-149-5p/HuR regulatory axis that supports GC progression.
Confidence score:

0.6867
Other information
Title:

circ_0044516 functions in the progression of gastric cancer by modulating MicroRNA-149-5p/HuR axis.

Journal: Molecular and cellular biochemistry
Published: 2022
PubMed ID: 33417162
Study type:

biological research
Data availability: The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -