| Expression pattern: |
UP |
| Associated gene: |
MAPK1, Ago2 |
| Associated microRNA: |
miR-556-5p |
| Biological function: |
promotes OS cell proliferation, colony formation ability, migration, and tumor growth in vivo |
| Molecular mechanism: |
circ_0020378 acts as a ceRNA that sponges miR-556-5p and promotes MAPK1 expression in OS progression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0020378/miR-556-5p/MAPK1 might be a novel target for OS intervention |
| Description: |
circ_0020378 is up-regulated in osteosarcoma tissues and cells and functions as an oncogenic circRNA. It promotes OS cell proliferation, colony formation, migration, and xenograft tumor growth by sponging miR-556-5p and relieving miR-556-5p-mediated repression of MAPK1. |
| Confidence score: |
0.7799 |