| Expression pattern: |
UP |
| Associated gene: |
MDM2 protein, FOXO3 protein |
| Associated microRNA: |
- |
| Biological function: |
promotes granulosa cell apoptosis under oxidative stress; knockdown attenuates H2O2-induced KGN cell apoptosis |
| Molecular mechanism: |
circFoxo3 interacts with MDM2 protein, reduces interactions between FOXO3 and MDM2, and regulates FOXO3 protein level by affecting MDM2-mediated ubiquitination and degradation |
| Biological pathway or process: |
apoptosis (promotes); ubiquitination (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Western Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Transfection; Annexin V/PI Flow Cytometry; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
potential therapeutic target for decreasing granulosa cell apoptosis in female ovarian disorders |
| Description: |
circFoxo3 is up-regulated in H2O2-treated granulosa cells and in a 3-NP-induced ovarian oxidative stress mouse model. It promotes granulosa cell apoptosis by interacting with MDM2 and regulating FOXO3 protein levels, thereby affecting MDM2-mediated FOXO3 ubiquitination and degradation. The study proposes circFoxo3 as a potential therapeutic target for reducing granulosa cell apoptosis in ovarian disorders. |
| Confidence score: |
0.7888 |