| Expression pattern: |
UP |
| Associated gene: |
SLC7A5 |
| Associated microRNA: |
miR-497-5p |
| Biological function: |
Promotes CRC cell proliferation, invasion, glutamine metabolism and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circ_0067717 functions as a miR-497-5p sponge to positively regulate SLC7A5 expression, thereby promoting CRC growth, invasion and glutamine metabolism. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); glutaminolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circ_0067717 expression was related to TNM stage, lymph node metastasis and tumor size of CRC patients; the circ_0067717/miR-497-5p/SLC7A5 pathway provided a potential molecular target for CRC treatment. |
| Description: |
circ_0067717 is up-regulated in colorectal cancer tissues and cell lines and is associated with advanced clinicopathological features. Functionally, it promotes CRC cell growth, invasion, glutamine metabolism and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ_0067717 acts as a sponge for miR-497-5p to upregulate SLC7A5. |
| Confidence score: |
0.7661 |