| Expression pattern: |
UP |
| Associated gene: |
CYLD, LATS2, NDRG2, TIMP3 |
| Associated microRNA: |
miR-181b |
| Biological function: |
promotes tumour growth, proliferation, migration, invasion and metastasis; promotes EMT |
| Molecular mechanism: |
circCSNK1G3 directly interacts with miR-181b and positively regulates miR-181b, leading to suppression of tumour suppressor genes (notably TIMP3) and thereby enhancing EMT |
| Biological pathway or process: |
EMT (promotes); metastasis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; BrdU; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
circCSNK1G3 is suggested as a potential diagnostic and prognostic biomarker in RCC |
| Description: |
circCSNK1G3 (hsa_circ_0001522), derived from CSNK1G3, is up-regulated in RCC and predominantly localized in the cytoplasm. It promotes RCC cell proliferation, migration/invasion and in vivo tumor growth by directly interacting with and up-regulating miR-181b, which suppresses tumor suppressors (notably TIMP3) and enhances EMT, leading to metastasis-related phenotypes. |
| Confidence score: |
0.8207 |