| Expression pattern: |
UP |
| Associated gene: |
KIF4A, Ago2 |
| Associated microRNA: |
miR-375 |
| Biological function: |
promotes TNBC cell proliferation and migration/metastasis; promotes tumor growth and lung metastasis in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-375 to regulate KIF4A expression; associated with Ago2-related RISC |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Cohort Study; Survival Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circKIF4A is positively associated with poorer survival of patients with TNBC and correlates with tumor size, lymph node metastasis and TNM stage |
| Description: |
circKIF4A (hsa_circ_0007255) is up-regulated in TNBC tissues and cell lines and its high expression is associated with worse patient survival. Functionally, circKIF4A promotes TNBC proliferation, migration/EMT and metastasis, and mechanistically acts as a ceRNA that sponges miR-375 to upregulate KIF4A. |
| Confidence score: |
0.8071 |