circRNA basic information
circBase ID: -
Name: hsa_circ_NFIX
Synonym: circRNA nuclear factor I X
Host Gene: nuclear factor I X
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
-
Disease MeSH ID:
D005910
Disease NCIt ID:
C3059
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum; peripheral blood

Cell lines:

LN229; U251; LN229-R; U251-R; LN229/luciferase

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: ABCG2
Associated microRNA: miR-132
Biological function: Exosomal CircNFIX enhances TMZ resistance in glioma, promotes tumor growth, increases cell migration and invasion, and represses apoptosis under TMZ exposure.
Molecular mechanism: Exosome-mediated transfer of CircNFIX promotes TMZ resistance at least partially by sponging miR-132; the CircNFIX/miR-132 axis regulates ABCG2 expression under TMZ exposure.
Biological pathway or process:

chemoresistance (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Cohort Study; ROC Analysis; Survival Analysis; Transfection; MTT; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model

Clinical significance:

Exosomal CircNFIX is associated with TMZ resistance and poor prognosis in glioma patients and showed diagnostic potential by ROC analysis.

Description:

CircNFIX is up-regulated in serum exosomes from TMZ-resistant glioma patients and in TMZ-resistant glioma cells, and high exosomal CircNFIX is associated with poor prognosis. Exosome-mediated transfer of CircNFIX promotes TMZ resistance by enhancing migration, invasion, ABCG2 expression, and tumor growth while suppressing apoptosis. Mechanistically, CircNFIX acts as a ceRNA by binding miR-132, and CircNFIX knockdown increases TMZ sensitivity in vitro and in vivo.

Confidence score:

0.7888

Other information
Title:

Exosome-mediated transfer of circRNA CircNFIX enhances temozolomide resistance in glioma.

Journal: Cancer letters
Published: 2020
PubMed ID: 32194140
Study type:

combined biological and clinical study

Data availability: All data that support the findings of this study are available from the corresponding authors upon reasonable request.
Code availability: -