circRNA basic information
circBase ID: hsa_circ_0001756
Name: hsa_circ_HIPK2
Synonym: circHIPK2
Host Gene: HIPK2
Genomic location(hg19): chr7:139415730-139416814:-
Genomic location(hg38): chr7:139715931-139717015:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021063
MONDO name: malignant colon neoplasm
Disease details: colon cancer
Disease DO ID:
 219
Disease MeSH ID:
 -
Disease NCIt ID:
 C9242
Disease ICD11 ID:
 1265576634
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

colon cancer tissues; adjacent normal colonic tissues
Cell lines:

HCT116; RKO; SW620; HT29; SW480; NCM460; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: RGMA, BMP/Smad signaling, HOXD10, AGO2, HIPK2
Associated microRNA: miR-373-3p
Biological function: circHIPK2 suppresses colon cancer cell proliferation, colony formation, migration, invasion, xenograft growth, and liver metastasis.
Molecular mechanism: circHIPK2 is transcriptionally regulated by HOXD10 and acts as a miR-373-3p sponge to relieve miR-373-3p-mediated repression of RGMA, activating BMP/Smad signaling.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); other pathway/process (promotes)
Detected method:
Q
H
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

Low circHIPK2 expression was associated with high tumor grade, high TNM stage, poor patient survival, and diagnostic value for colon cancer screening; circHIPK2 may serve as a diagnostic/prognostic biomarker.
Description:

circHIPK2 is a human HIPK2-derived circRNA downregulated in colon cancer. It suppresses colon cancer proliferation, migration, invasion, tumor growth, and metastasis by sponging miR-373-3p, thereby increasing RGMA and activating BMP/Smad signaling. Clinically, low circHIPK2 expression is associated with higher tumor grade/stage and poorer survival, supporting its potential diagnostic and prognostic value.
Confidence score:

0.8999
Other information
Title:

Circular RNA circHIPK2 inhibits colon cancer cells through miR-373-3p/RGMA axis.

Journal: Cancer letters
Published: 2024
PubMed ID: 38762192
Study type:

combined biological and clinical study
Data availability: GSE138589; GSE126094; Supplemental Information file; Supplemental Table 1; Supplemental Table 2; Supplemental Table 3
Code availability: -