| Expression pattern: |
UP |
| Associated gene: |
SLC1A5 |
| Associated microRNA: |
miR-876-3p |
| Biological function: |
Promotes TNBC cell proliferation, survival, migration, invasion, glutamine metabolism, and tumor growth; circ_0062558 knockdown inhibits these malignant phenotypes and enhances apoptosis. |
| Molecular mechanism: |
circ_0062558 functions as a ceRNA/miRNA sponge for miR-876-3p, thereby positively regulating SLC1A5 expression and promoting TNBC progression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); glutaminolysis (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
circ_0062558 may serve as a therapeutic target for TNBC patients. |
| Description: |
circ_0062558 is up-regulated in TNBC tissues and cells and acts as an oncogenic circRNA. It promotes proliferation, survival, migration, invasion, glutamine metabolism, and xenograft tumor growth by sponging miR-876-3p and increasing SLC1A5 expression, suggesting potential therapeutic relevance. |
| Confidence score: |
0.8443 |