circRNA basic information
circBase ID: hsa_circ_0000395
Name: hsa_circ_SLC38A1
Synonym: Circ_0000395
Host Gene: SLC38A1
Genomic location(hg19): chr12:46622935-46624417:-
Genomic location(hg38): chr12:46229152-46230634:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tumor tissues; adjacent normal tissues; tumor tissues
Cell lines:

HCT116; SW480; NCM460
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MYO6, AGO2, MRP1, MDR1
Associated microRNA: miR-153-5p
Biological function: Promotes CRC cell proliferation, migration, invasion, tumor growth, metastasis, oxaliplatin resistance, and inhibits apoptosis.
Molecular mechanism: circ_0000395 functions as a miR-153-5p sponge to regulate MYO6 expression, thereby promoting CRC progression and oxaliplatin resistance; MRP1 and MDR1 drug-resistance markers are reduced after circ_0000395 knockdown.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); drug resistance (promotes); chemoresistance (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; CCK8; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0000395 expression was associated with lower survival rate in CRC patients and may act as an effective therapeutic target for CRC.
Description:

circ_0000395 is up-regulated in colorectal cancer tissues and cells, and high expression is associated with poorer patient survival. Functionally, it promotes CRC cell growth, migration, invasion, tumor growth, metastasis and oxaliplatin resistance while suppressing apoptosis. Mechanistically, circ_0000395 acts as a miR-153-5p sponge to regulate MYO6, contributing to CRC progression and chemoresistance.
Confidence score:

0.8443
Other information
Title:

Circ_0000395 promotes cell growth, metastasis and oxaliplatin resistance by regulating miR-153-5p/MYO6 in colorectal cancer.

Journal: Pathology, research and practice
Published: 2024
PubMed ID: 39038387
Study type:

combined biological and clinical study
Data availability: Supplementary data associated with this article can be found in the online version at doi:10.1016/j.prp.2024.155476.
Code availability: -