circRNA basic information
circBase ID: hsa_circ_0003011
Name: hsa_circ_RFWD2
Synonym: -
Host Gene: RFWD2
Genomic location(hg19): chr1:176132004-176145143:-
Genomic location(hg38): chr1:176162868-176176007:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma / LUAD
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

cerebrospinal fluid / CSF; plasma; tumor tissue
Cell lines:

A549; HCC827
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UN
Associated gene: FZD
Associated microRNA: -
Biological function: promotes cell proliferation; promotes invasion
Molecular mechanism: Predicted to be involved in a circRNA-miRNA-mRNA network and associated with WNT signaling pathway target genes (e.g., FZD).
Biological pathway or process:

Wnt/beta-catenin (other); proliferation (promotes); invasion (promotes); ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Transfection; MTT; Transwell Assay; Colony Formation Assay; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Included in 60 CSF-circRNAs that were identified and associated with shorten overall survival.
Description:

hsa_circ_0003011 was selected from CSF circRNAs associated with WNT signaling. In A549 cells, shRNA-mediated knockdown reduced proliferation and invasion, suggesting a pro-tumor role in lung adenocarcinoma with brain metastases.
Confidence score:

0.7325
Other information
Title:

Clinical utility of cerebrospinal fluid-derived circular RNAs in lung adenocarcinoma patients with brain metastases.

Journal: Journal of translational medicine
Published: 2022
PubMed ID: 35123506
Study type:

combined biological and clinical study
Data availability: The online version contains supplementary material available at 10.1186/s12967-022-03274-1.
Code availability: -