| Expression pattern: |
UP |
| Associated gene: |
YTHDC1, IGF2BP1, IGF2BP2, IGF2BP3, VEGFA mRNA |
| Associated microRNA: |
- |
| Biological function: |
circPAK2 enhances migration, invasion, lymphangiogenesis, angiogenesis, EMT, and metastasis of gastric cancer, but does not significantly affect proliferation. |
| Molecular mechanism: |
m6A-modified circPAK2 is exported from nucleus to cytoplasm by YTHDC1 in an m6A-dependent manner; cytoplasmic circPAK2 interacts with IGF2BPs and VEGFA mRNA to form a circPAK2/IGF2BPs/VEGFA complex that stabilizes VEGFA mRNA and promotes VEGFA signaling. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); EMT (promotes); angiogenesis (promotes); metastasis (promotes); m6A modification (promotes); mRNA stability (promotes); VEGF/VEGFR (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; IF (Immunofluorescence); MeRIP / MeRIP-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); ELISA; Western Blot; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circPAK2 expression is positively associated with LN metastasis and poor prognosis; circPAK2 may serve as a predictor of LN metastasis, prognostic biomarker, and therapeutic target for gastric cancer. |
| Description: |
circPAK2 is a human PAK2-derived circRNA that is upregulated in gastric cancer tissues and metastatic lymph node tissues. It promotes gastric cancer migration, invasion, EMT, angiogenesis, lymphangiogenesis, and lymph node metastasis through an m6A-dependent YTHDC1-mediated nuclear export mechanism and a cytoplasmic circPAK2/IGF2BPs/VEGFA complex that stabilizes VEGFA mRNA. High circPAK2 expression predicts LN metastasis and poor OS/DFS, supporting its potential use as a prognostic biomarker and therapeutic target. |
| Confidence score: |
0.8999 |