| Expression pattern: |
UP |
| Associated gene: |
IGF2, AGO2 |
| Associated microRNA: |
miR-665 |
| Biological function: |
promotes HCC cell proliferation and colony formation, promotes cell cycle progression, inhibits apoptosis, and promotes in vivo tumor growth |
| Molecular mechanism: |
ceRNA mechanism: circTMEM45A sponges miR-665 to relieve repression of IGF2 |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
higher circTMEM45A in HCC tissues (AUC=0.888) and high expression correlates with tumor size, TNM stage, vascular invasion and poor overall survival; detectable and higher in serum exosomes from HCC patients |
| Description: |
circTMEM45A (hsa_circ_0066659), derived from TMEM45A, is up-regulated in HCC tissues, HCC cell lines, and serum exosomes from HCC patients. It promotes HCC malignant phenotypes and xenograft tumor growth mainly by acting as a cytoplasmic ceRNA that sponges miR-665, thereby upregulating IGF2, and its high expression is associated with poor overall survival and diagnostic potential. |
| Confidence score: |
0.8824 |