| Expression pattern: |
UP |
| Associated gene: |
STAT3, phospho-STAT3 |
| Associated microRNA: |
miR-93-5p |
| Biological function: |
Mediates Nd 2 O 3-induced inflammation by increasing IL-6 and IL-8, inhibits proliferation of Nd 2 O 3-induced 16HBE cells, and promotes G0/G1 cell cycle arrest; it was not involved in Nd 2 O 3-induced cell apoptosis. |
| Molecular mechanism: |
circ_0039411 acts as a ceRNA by sponging miR-93-5p, thereby regulating STAT3 phosphorylation and mediating Nd 2 O 3-induced inflammation and dysfunction. |
| Biological pathway or process: |
inflammation (promotes); proliferation (inhibits); cell cycle (inhibits); ceRNA regulation (other); JAK/STAT (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; Transfection; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
May serve as a molecular target for prevention of Nd 2 O 3-induced health damage and support risk/hazard assessment. |
| Description: |
circ_0039411 is an MMP2-derived human circRNA upregulated in Nd 2 O 3-treated bronchial epithelial cells. It promotes inflammatory cytokine production and anti-proliferative effects by arresting cells at G0/G1, functioning as a miR-93-5p sponge that enhances STAT3 phosphorylation. |
| Confidence score: |
0.6941 |