| Expression pattern: |
UP |
| Associated gene: |
HMGB2 |
| Associated microRNA: |
miR-637 |
| Biological function: |
Promotes osteosarcoma cell growth/proliferation, invasion and migration; suppresses apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
circ_UBAP2 functions as a ceRNA/miRNA sponge for miR-637, thereby upregulating HMGB2. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
High circ_UBAP2 expression was associated with distant metastasis and clinical stage (TNM stage) in osteosarcoma. |
| Description: |
circ_UBAP2 is up-regulated in osteosarcoma tissues and cell lines and promotes malignant behaviors (proliferation, migration, invasion) while inhibiting apoptosis. It mainly localizes to the cytoplasm and acts as a ceRNA that sponges miR-637 to relieve repression of HMGB2, thereby promoting osteosarcoma progression in vitro and in xenograft models. |
| Confidence score: |
0.7795 |