| Expression pattern: |
UP |
| Associated gene: |
Homeobox B6 (HOXB6), HOXB6, CCL3, CXCL11, CXCL1 |
| Associated microRNA: |
miR-196a-5p |
| Biological function: |
Promotes OS cell proliferation, migration, invasion, tumor growth, metastasis, EMT, and cancer stem cell stemness/self-renewal. |
| Molecular mechanism: |
ceRNA mechanism: cytoplasmic hsa_circ_0007031 sponges miR-196a-5p, thereby relieving miR-196a-5p-mediated suppression of HOXB6; HOXB6 modulates chemokines in the cytokine-cytokine receptor interaction signaling pathway. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); stemness (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
May be a promising therapeutic target and possible diagnostic biomarker for OS. |
| Description: |
In this study, hsa_circ_0007031 is up-regulated in osteosarcoma tissues and cell lines and functions as an oncogenic circRNA. It promotes proliferation, migration, invasion, EMT, tumor growth, metastasis, and stem-like properties by sponging miR-196a-5p to increase HOXB6 expression. The hsa_circ_0007031/miR-196a-5p/HOXB6 axis further modulates chemokines in the cytokine-cytokine receptor interaction pathway and is proposed as a potential therapeutic or diagnostic target in OS. |
| Confidence score: |
0.8256 |