| Expression pattern: |
UP |
| Associated gene: |
MAP4K3, ERK1/2, JNK, p38 |
| Associated microRNA: |
miR‐142‐3p |
| Biological function: |
Inhibits proliferation, migration, invasion and angiogenesis; promotes cell cycle arrest and apoptosis when knocked down; promotes tumor growth in vivo. |
| Molecular mechanism: |
circ-RAD23B acts as a miR-142-3p sponge to increase MAP4K3 expression; associated with inactivation of MAPK signaling (reduced p-ERK1/2, p-JNK, p-p38) upon circ-RAD23B knockdown. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); cell cycle (promotes); MAPK (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Tube Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
High circ-RAD23B expression was linked to low survival of NSCLC patients. |
| Description: |
circ-RAD23B is up-regulated in NSCLC and functions as an oncogenic circRNA. Its knockdown suppresses malignant phenotypes and tumor growth by sponging miR-142-3p to relieve repression of MAP4K3, with downstream reduction of MAPK pathway activation (p-ERK1/2, p-JNK, p-p38). High circ-RAD23B expression correlates with poorer patient survival. |
| Confidence score: |
0.7133 |