circRNA basic information
circBase ID: hsa_circ_0001806
Name: hsa_circ_CSPP1
Synonym: circCSPP1
Host Gene: CSPP1
Genomic location(hg19): chr8:68018139-68028357:+
Genomic location(hg38): chr8:67105904-67116122:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021063
MONDO name: malignant colon neoplasm
Disease details: colon cancer
Disease DO ID:
 219
Disease MeSH ID:
 -
Disease NCIt ID:
 C9242
Disease ICD11 ID:
 1265576634
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

colon cancer tissues; adjacent normal tissues; tumor tissues
Cell lines:

HFC; SW620; SW480; LOVO; HCT116; DLD-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ROCK1, ZEB1
Associated microRNA: miR-431
Biological function: promotes proliferation, migration, invasion, tumor growth and metastasis in colon cancer
Molecular mechanism: Acts as a competing endogenous RNA by sponging miR-431 to regulate ROCK1 and ZEB1 expression; associated with cyclin D1/CDK4/Rb signaling and EMT-related markers.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); cell cycle (other); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; RNase R Treatment; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Cell Cycle Assay; In Vivo Animal Model
Clinical significance:

circCSPP1 is upregulated in colon cancer tissues and may be a potential biomarker and a diagnostic and therapeutic target.
Description:

circCSPP1 (hsa_circ_0001806), derived from CSPP1 exons 8-11, is upregulated in colon cancer tissues and cell lines and mainly localizes to the cytoplasm. It promotes colon cancer proliferation, migration, invasion, tumor growth and metastasis by sponging miR-431, thereby upregulating ROCK1 and ZEB1 and influencing cell-cycle and EMT-related proteins.
Confidence score:

0.8164
Other information
Title:

Circular RNA circCSPP1 promotes the occurrence and development of colon cancer by sponging miR-431 and regulating ROCK1 and ZEB1.

Journal: Journal of translational medicine
Published: 2022
PubMed ID: 35101080
Study type:

combined biological and clinical study
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -