circRNA basic information
circBase ID: hsa_circ_0000005
Name: hsa_circ_CDK11A
Synonym: circ_002136
Host Gene: CDK11A
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

hCMEC/D3; U87MG; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FUS, SOX13, SPON2
Associated microRNA: miR-138-5p
Biological function: promotes angiogenesis (increases viability, migration and tube formation) in glioma-exposed endothelial cells
Molecular mechanism: FUS binds and promotes circ_002136 generation; circ_002136 sponges miR-138-5p in RISC to relieve repression on SOX13; SOX13 transcriptionally activates SPON2 and also activates FUS forming a positive feedback loop
Biological pathway or process:

angiogenesis (promotes); proliferation (promotes); migration (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

circ_002136 is up-regulated in glioma-exposed endothelial cells and promotes angiogenesis-related phenotypes (viability, migration, tube formation). Mechanistically, it is promoted by FUS binding and acts as a ceRNA by sponging miR-138-5p to increase SOX13, which transcriptionally activates SPON2 and also enhances FUS to form a positive feedback loop.
Confidence score:

0.721
Other information
Title:

FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2019
PubMed ID: 30736838
Study type:

biological research
Data availability: Additional file 1: Figure S1; Additional file 2: Figure S2
Code availability: -