circRNA basic information
circBase ID: hsa_circ_0000936
Name: hsa_circ_SHKBP1
Synonym: circSHKBP1 / circ-SHKBP1
Host Gene: SHKBP1
Genomic location(hg19): chr19:41089303-41089623:+
Genomic location(hg38): chr19:40583397-40583717:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; normal adjacent tissue; serum; serum exosomes
Cell lines:

HBE; A549; PC9; H1650; H1299; THP-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PKM2, Ago2, HIF-1alpha
Associated microRNA: miR-1294
Biological function: Promotes NSCLC cell proliferation, migration, invasion, stemness, glycolysis, macrophage M2 polarization and recruitment; suppresses CD8+ T cell cytokine secretion; promotes tumor growth and metastasis in vivo.
Molecular mechanism: Exosomal circSHKBP1 acts as a ceRNA to sponge miR-1294 and upregulate PKM2, enhancing glycolysis in a PKM2/HIF-1alpha-dependent manner, thereby promoting malignant phenotypes and shaping the tumor microenvironment (M2 polarization, macrophage recruitment).
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); stemness (promotes); glycolysis (promotes); HIF-1 signaling (promotes); macrophage polarization (promotes); immune regulation (other); ceRNA regulation (promotes); metastasis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); ROC Analysis; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Serum exosomal circSHKBP1 shows diagnostic value for NSCLC (AUC=0.853) and its expression levels are related to lymphatic metastasis and TNM stage; overexpression is associated with poor prognosis.
Description:

circSHKBP1 (hsa_circ_0000936), enriched in NSCLC-derived exosomes, is upregulated in NSCLC and promotes proliferation, migration/invasion, stemness, glycolysis, and in vivo tumor growth/metastasis. Mechanistically, it sponges miR-1294 to upregulate PKM2 and activate PKM2/HIF-1alpha-dependent glycolysis, fostering M2 macrophage polarization/recruitment and suppressing CD8+ T-cell cytokine secretion. Serum exosomal circSHKBP1 shows potential diagnostic value and correlates with advanced disease features and poor prognosis.
Confidence score:

0.8818
Other information
Title:

Exosomal circSHKBP1 participates in non-small cell lung cancer progression through PKM2-mediated glycolysis.

Journal: Molecular therapy oncolytics
Published: 2022
PubMed ID: 35229026
Study type:

combined biological and clinical study
Data availability: GEO: GSE158695; https://doi.org/10.1016/j.omto.2022.01.012
Code availability: -