| Expression pattern: |
DN |
| Associated gene: |
PTEN, AKT, Ago2 |
| Associated microRNA: |
miR-141-3p |
| Biological function: |
Suppresses hepatic stellate cell activation by inhibiting proliferation, inducing apoptosis, causing cell cycle arrest, and reducing pro-fibrotic markers (alpha-SMA, collagen I). |
| Molecular mechanism: |
Acts as an endogenous sponge for miR-141-3p to increase PTEN and inhibit AKT activation (reduced p-AKT/AKT), including exosome-mediated transfer from MSCs to HSCs. |
| Biological pathway or process: |
PI3K/AKT (inhibits); proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); fibrosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low levels of serum circDIDO1 in exosome were correlated with liver failure, and serum exosomal circDIDO1 had a well diagnostic value for liver fibrosis in liver failure patients. |
| Description: |
circDIDO1 is a DIDO1-derived cytoplasmic circRNA that is delivered via MSC exosomes to hepatic stellate cells, where it sponges miR-141-3p to increase PTEN and inhibit AKT activation, thereby suppressing HSC activation and fibrotic phenotypes. Serum exosomal circDIDO1 is reduced in liver failure patients and shows diagnostic value for liver fibrosis in this context. |
| Confidence score: |
0.8735 |