circRNA basic information
circBase ID: hsa_circ_0001839
Name: hsa_circ_KDM4C
Synonym: circKDM4C
Host Gene: KDM4C
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; matched adjacent normal tissue; xenograft tumors; lungs
Cell lines:

MCF10A; MCF-7; MDA-MB-231; MDA-MB-468; MDA-MB-231/DOX; HEK293T; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: PBLD, AGO2
Associated microRNA: miR-548p
Biological function: circKDM4C suppresses breast cancer proliferation, migration, invasion, EMT, angiogenic tube formation, metastasis, tumor growth, and doxorubicin resistance, while promoting apoptosis and inhibiting cell cycle progression.
Molecular mechanism: circKDM4C acts as a ceRNA/miR-548p sponge and protects PBLD from miR-548p-mediated degradation, thereby suppressing malignant phenotypes and doxorubicin resistance in breast cancer.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); apoptosis (promotes); cell cycle (inhibits); EMT (inhibits); angiogenesis (inhibits); ceRNA regulation (promotes); drug resistance (inhibits); chemoresistance (inhibits)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Colony Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining
Clinical significance:

Lower circKDM4C expression was associated with poor prognosis and metastasis in breast cancer; circKDM4C may serve as a prognostic biomarker and may predict chemoresponse.
Description:

circKDM4C is a KDM4C-derived human circRNA that is downregulated in breast cancer tissues, metastatic breast cancer tissues, breast cancer cell lines, and doxorubicin-resistant cells. It functions as a tumor suppressor by sponging miR-548p and upregulating PBLD, thereby inhibiting proliferation, EMT, migration, invasion, metastasis, angiogenic tube formation, and doxorubicin resistance while promoting apoptosis. Clinically, low circKDM4C expression is associated with lymphatic metastasis and worse overall survival, suggesting prognostic and therapeutic relevance.
Confidence score:

0.8868
Other information
Title:

circKDM4C suppresses tumor progression and attenuates doxorubicin resistance by regulating miR-548p/PBLD axis in breast cancer.

Journal: Oncogene
Published: 2019
PubMed ID: 31406252
Study type:

combined biological and clinical study
Data availability: Table S1; Table S2; Table S3; Table S4; Table S5; TCGA; GEO; Circular RNA Interactome database
Code availability: -