| Expression pattern: |
UP |
| Associated gene: |
HK2, Ago2 |
| Associated microRNA: |
miR-653 |
| Biological function: |
Accelerates hypoxia-induced glycolysis, migration, and invasion in LUAD cells; circ_0061140 knockdown inhibits these malignant phenotypes under hypoxia. |
| Molecular mechanism: |
Acts as a ceRNA (miRNA sponge) for miR-653 to increase HK2 expression, promoting hypoxia-driven LUAD progression. |
| Biological pathway or process: |
glycolysis (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Wound Healing Assay; Transwell Assay; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
High circ_0061140 level is associated with poor survival rate in LUAD patients; potential therapeutic and diagnostic target. |
| Description: |
circ_0061140 is up-regulated in LUAD tissues/cells (further increased under hypoxia) and promotes hypoxia-induced glycolysis, migration, and invasion. Mechanistically, it sponges miR-653 to de-repress HK2, and high circ_0061140 expression correlates with poorer patient survival. |
| Confidence score: |
0.7507 |