| Expression pattern: |
UP |
| Associated gene: |
CARM1 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
Promotes high glucose-induced retinal pigment epithelial cell injury by promoting inflammation, apoptosis, and oxidative stress and inhibiting proliferation. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-338-3p to upregulate CARM1 (ceRNA-like mechanism). |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Clinical Sample Validation; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
Upregulated in serum of DR patients compared with normal controls. |
| Description: |
circ-ADAM9 is upregulated in DR patient serum and in high glucose-treated ARPE-19 cells. Functionally, circ-ADAM9 promotes HG-induced RPE cell injury (inflammation, apoptosis, oxidative stress) and suppresses proliferation. Mechanistically, it sponges miR-338-3p to increase CARM1 expression (circ-ADAM9/miR-338-3p/CARM1 axis). |
| Confidence score: |
0.7795 |