circRNA basic information
circBase ID: -
Name: hsa_circ_ZFR
Synonym: circZFR
Host Gene: ZFR
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues

Cell lines:

L02; Huh7; MHCC97L; Huh7/DDP; MHCC97L/DDP

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: STAT3, NF-kappaB
Associated microRNA: -
Biological function: Promotes cisplatin (DDP) chemoresistance, promotes tumor growth/proliferation, and inhibits apoptosis in HCC.
Molecular mechanism: CAFs-derived exosomes deliver circZFR to HCC cells, leading to inhibition of STAT3/NF-kappaB signaling (reduced STAT3 and NF-kappaB phosphorylation), thereby enhancing DDP resistance.
Biological pathway or process:

NF-kappaB (inhibits); JAK/STAT (inhibits); proliferation (promotes); apoptosis (inhibits); drug resistance (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; TUNEL; IHC (Immunohistochemistry)

Clinical significance:

-

Description:

circZFR is up-regulated in cisplatin-resistant HCC cells and in CAF-derived exosomes. CAF exosomes transfer circZFR to HCC cells, suppress STAT3/NF-kappaB phosphorylation, increase cell viability, reduce apoptosis, and thereby promote cisplatin (DDP) chemoresistance and tumor growth in vitro and in a nude-mouse xenograft model.

Confidence score:

0.5442

Other information
Title:

Cancer-associated fibroblast exosomes promote chemoresistance to cisplatin in hepatocellular carcinoma through circZFR targeting signal transducers and activators of transcription (STAT3)/ nuclear factor -kappa B (NF-kappaB) pathway.

Journal: Bioengineered
Published: 2022
PubMed ID: 35139763
Study type:

biological research

Data availability: The datasets analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -