| Expression pattern: |
UP |
| Associated gene: |
CCDC66, AGO2 |
| Associated microRNA: |
hsa-miR-4259 |
| Biological function: |
Promotes breast cancer cell proliferation and stem cell spheroidization/mammosphere formation; slightly reduces apoptosis without significant change. |
| Molecular mechanism: |
circ-CCDC66 functions as a molecular sponge for hsa-miR-4259 and upregulates CCDC66 expression, forming a circ-CCDC66/miR-4259/CCDC66 regulatory axis. |
| Biological pathway or process: |
proliferation (promotes); stemness (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; RNA-seq; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
May serve as a potential therapeutic target for breast cancer treatment. |
| Description: |
circ-CCDC66 is up-regulated in breast cancer tissues and cell lines. It promotes breast cancer cell proliferation and mammosphere/stem cell spheroidization, at least partly by sponging hsa-miR-4259 and increasing CCDC66 expression. The study proposes the circ-CCDC66/miR-4259/CCDC66 axis as a potential therapeutic target in breast cancer. |
| Confidence score: |
0.8111 |