| Expression pattern: |
UP |
| Associated gene: |
HECTD1, GPX4 |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, invasion, and migration; decreases apoptosis; inhibits ferroptosis in gastric cancer cells |
| Molecular mechanism: |
hsa_circ_0002301 encodes HECTD1-463aa, which binds HECTD1 and inhibits HECTD1-mediated GPX4 ubiquitination/degradation, thereby suppressing ferroptosis |
| Biological pathway or process: |
ferroptosis (inhibits); ubiquitination (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; Co-IP; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Bioinformatics Analysis; circRNA-seq; RNA-seq |
| Clinical significance: |
Patients with higher levels of hsa_circ_0002301 in gastric cancer tissues experienced significantly shorter overall survival; high expression correlates with large tumor size, lymphatic invasion, high TNM stage, and hematogenous metastasis. |
| Description: |
hsa_circ_0002301 is up-regulated in gastric cancer and promotes malignant phenotypes. It inhibits ferroptosis by encoding the peptide HECTD1-463aa, which binds HECTD1 and reduces HECTD1-mediated GPX4 ubiquitination/degradation, stabilizing GPX4. High hsa_circ_0002301 expression is associated with worse overall survival and more aggressive clinicopathological features. |
| Confidence score: |
0.8949 |