| Expression pattern: |
UP |
| Associated gene: |
LASP1, Ago2 |
| Associated microRNA: |
miR-485-5p |
| Biological function: |
promotes proliferation, invasion, stemness, and tumor growth; inhibits apoptosis |
| Molecular mechanism: |
ceRNA mechanism: circ_0001535 sponges miR-485-5p to upregulate LASP1 |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); invasion (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Colony Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0001535 had a poor overall survival rate. |
| Description: |
circ_0001535 is up-regulated in colorectal cancer and promotes malignant phenotypes (proliferation, invasion, stemness) while suppressing apoptosis. Mechanistically, it mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-485-5p, thereby increasing LASP1 expression and enhancing tumor growth in vivo. |
| Confidence score: |
0.8712 |