circRNA basic information
circBase ID: hsa_circ_0020123
Name: hsa_circ_PDZD8
Synonym: circular RNA_0020123 / circ_0020123
Host Gene: PDZD8
Genomic location(hg19): chr10:119042605-119049859:-
Genomic location(hg38): chr10:117283094-117290348:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue samples; adjacent normal lung tissue samples; serum
Cell lines:

A549; H1299; PC-9; 16HBE; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CORO1C, Ago2
Associated microRNA: miR-512-3p
Biological function: promotes proliferation, migration, invasion, angiogenesis and autophagy; inhibits apoptosis; promotes tumor growth in vivo
Molecular mechanism: ceRNA mechanism: circ_0020123 sponges miR-512-3p to upregulate CORO1C
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); autophagy (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

High levels of circ_0020123 are closely correlated with advanced TNM tumor stage in NSCLC patients; circ_0020123 is upregulated in serum samples of NSCLC patients and positively correlated with TNM staging
Description:

Circ_0020123 is up-regulated in NSCLC tissues, serum, and NSCLC cell lines and mainly localizes to the cytoplasm. Functionally, it promotes malignant phenotypes (proliferation, migration/invasion, angiogenesis, and autophagy) and suppresses apoptosis; in vivo knockdown inhibits xenograft growth. Mechanistically, circ_0020123 acts as a ceRNA that sponges miR-512-3p to increase CORO1C expression.
Confidence score:

0.8039
Other information
Title:

Circ_0020123 plays an oncogenic role in non-small cell lung cancer depending on the regulation of miR-512-3p/CORO1C.

Journal: Thoracic cancer
Published: 2022
PubMed ID: 35388975
Study type:

combined biological and clinical study
Data availability: -
Code availability: -