| Expression pattern: |
UP |
| Associated gene: |
STIL, GLi1, CDK1, Cyclin B1, SUFU |
| Associated microRNA: |
miR-144 |
| Biological function: |
promotes proliferation, migration and invasion; inhibits apoptosis; promotes cell cycle progression; promotes malignant transformation of HPDE cells via exosomal transfer |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-144 to regulate STIL (and downstream GLi1, CDK1, Cyclin B1), affecting cell cycle and malignant phenotypes; can be transferred via SW1990-derived exosomes to HPDE cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Luciferase Reporter Assay; RNA Pull-Down; FISH / smFISH; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; In Vivo Animal Model; TUNEL; Bioinformatics Analysis; Clinical Sample Validation; ROC Analysis |
| Clinical significance: |
Diagnostic value by ROC (AUC=0.8944); correlated with distant metastasis and tumor volume; associated with poor prognosis (as stated by authors). |
| Description: |
hsa_circ_0000069 is upregulated in pancreatic cancer and functions as an oncogenic circRNA. It sponges miR-144 to upregulate STIL (with effects on GLi1 and cell-cycle regulators), promoting proliferation, migration/invasion and cell-cycle progression while suppressing apoptosis. SW1990-derived exosomes can transfer hsa_circ_0000069 to HPDE cells, and reducing exosomal hsa_circ_0000069 suppresses HPDE malignant transformation. |
| Confidence score: |
0.7861 |