circRNA basic information
circBase ID: -
Name: hsa_circ_CCDC66
Synonym: -
Host Gene: CCDC66
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

colorectal tumors; matched healthy tissue
Cell lines:

RKO; HCT-116; SW620; SW480; NCM460
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: MDM4
Associated microRNA: miR-370
Biological function: promotes proliferation, migration, and invasion of CRC cells
Molecular mechanism: acts as a ceRNA/miRNA sponge for miR-370 to upregulate MDM4
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

-
Description:

circCCDC66 is up-regulated in colorectal cancer tissues and CRC cell lines and functions as an oncogenic circRNA. It mainly localizes to the cytoplasm, sponges miR-370, and thereby increases MDM4 expression, promoting CRC cell proliferation, migration, and invasion.
Confidence score:

0.6777
Other information
Title:

Circular RNA CCDC66 Improves Murine Double Minute 4 (MDM4) Expression through Targeting miR-370 in Colorectal Cancer.

Journal: Computational and mathematical methods in medicine
Published: 2022
PubMed ID: 35069793
Study type:

combined biological and clinical study
Data availability: All the data during the current study were available from the corresponding author on reasonable request.
Code availability: -