| Expression pattern: |
UP |
| Associated gene: |
GLI2, hedgehog |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
promotes PCa cell proliferation and migration; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a molecular sponge (ceRNA) for miR-338-3p, thereby regulating GLI2 and activating hedgehog signaling to promote PCa progression. |
| Biological pathway or process: |
Hedgehog (promotes); proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RT-qPCR; ISH (In Situ Hybridization); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; RNA-seq; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; Clinical Sample Validation |
| Clinical significance: |
hsa_circ_0070512 expression was associated with clinicopathological features (tumor T stage) and proposed as a diagnostic and therapeutic target for PCa. |
| Description: |
hsa_circ_0070512 is up-regulated in prostate cancer tissues and cell lines, mainly localized in the cytoplasm, and promotes PCa proliferation and migration. It functions as a ceRNA by sponging miR-338-3p, increasing GLI2 expression and activating hedgehog signaling, and it enhances tumor growth in a PC3 xenograft model. |
| Confidence score: |
0.8192 |