| Expression pattern: |
UP |
| Associated gene: |
HIF-1alpha, VHL |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, migration, invasion, metastasis and tumorigenesis; inhibits apoptosis; promotes glycolysis under hypoxic stress |
| Molecular mechanism: |
HIF-1alpha transcriptionally induces Hsa_circ_0000566; Hsa_circ_0000566 binds HIF-1alpha and VHL, disrupts VHL-HIF-1alpha interaction, inhibits HIF-1alpha ubiquitination/degradation, stabilizes HIF-1alpha, forming a positive feedback loop to enhance glycolysis |
| Biological pathway or process: |
glycolysis (promotes); Warburg effect (promotes); ubiquitination (inhibits); metastasis (promotes); invasion (promotes); proliferation (promotes); apoptosis (inhibits) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
Hsa_circ_0000566 expression is higher in osteosarcoma tissues than in chondroma controls, suggesting potential as a therapeutic target |
| Description: |
Hsa_circ_0000566 is upregulated in osteosarcoma, especially under hypoxia, and promotes malignant phenotypes and glycolysis. Mechanistically, HIF-1alpha transcriptionally induces Hsa_circ_0000566, while Hsa_circ_0000566 binds HIF-1alpha and VHL to block VHL-mediated ubiquitination, stabilizing HIF-1alpha and forming a positive feedback loop that enhances the Warburg effect. |
| Confidence score: |
0.8748 |