circRNA basic information
circBase ID: hsa_circ_0060967
Name: hsa_circ_RAE1
Synonym: circ_0060967 / circRAE1
Host Gene: RAE1
Genomic location(hg19): chr20:55931552-55943868:+
Genomic location(hg38): chr20:57356496-57368812:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; paired normal tissues adjacent to cancer; xenograft tumor tissues
Cell lines:

NHBE; NCl-H1395; A549; H1975; H1299; Calu3
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: UBN2 / ubinuclein-2
Associated microRNA: miR-660-3p
Biological function: Facilitates NSCLC cell viability, proliferation, migration, invasion, and in vivo growth; acts as an oncogene of NSCLC.
Molecular mechanism: circ_0060967 acts as a ceRNA by sponging miR-660-3p to enhance UBN2 expression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Transwell Assay; In Vivo Animal Model; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

High circ_0060967 expression implied grim prognosis and was related to poor TNM stage and larger tumor size; it might be a promising target for NSCLC treatment.
Description:

circ_0060967 is up-regulated in NSCLC tissues and cell lines, and high expression is associated with grim prognosis, poor TNM stage, and larger tumor size. Functionally, circ_0060967 promotes NSCLC cell viability, proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, it is mainly cytoplasmic and promotes malignant phenotypes by sponging miR-660-3p to increase UBN2 expression.
Confidence score:

0.7756
Other information
Title:

Circ_0060967 facilitates proliferation, migration, and invasion of non-small-cell lung cancer cells by sponging miR-660-3p/UBN2.

Journal: Molecular and cellular biochemistry
Published: 2023
PubMed ID: 36229758
Study type:

combined biological and clinical study
Data availability: All data can be obtained by contacting the corresponding author.
Code availability: -